Pdf biodiesel production from heterotrophic microalgal. These include methane produced by anaerobic digestion of the algal biomass6 biodiesel derived from microalgal oil11,12, and. Biofuels from microalgae li 2008 biotechnology progress. Among 89 strains, only five were capable of growing under heterotrophic conditions in liquid cultures and chlorella sp. Although numerous reports are available on the production of biodiesel from vegetable oils of terraneous oil plants, such as soybean, sun. This revision focuses in the biotechnological potential and viability of biodiesel production from microalgae. Crude microalgal oil was employed in this study because the oil purification processes impose extra expenses and increase the final cost of the product. An integrated approach of biodiesel production from heterotrophic chlorella protothecoides focused on scaling up fermentation in bioreactors was reported in this study. B1 in which three fatty acid molecules are esterified with a molecule of glycerol. Microalgae can provide several different types of renewable biofuels.
Jun 30, 2016 currently, biodiesel production from microalgal biomass has drawn an immense importance with the potential to exclude highquality agricultural land use and food safekeeping issues. Investigations on microalgal oil production from arthrospira. In this study, biodiesel production from microalgal oil by microwaveassisted transesterification was carried out to investigate its efficiency. Based on the quality and quantity standard of biodiesel, the optimized temperature and the reaction time for in situ biodiesel production from microalgal biomass were 90c and 2 hours. Frontiers industrial fermentation of auxenochlorella. Biodiesel comparable to conventional diesel was obtained from. A similar fame profile was also found for both biodiesel samples, comprising c16. Comparison of autotrophic and heterotrophic cultivations. Through substrate feeding and fermentation process controls, the cell density of c.
They have been investigated for the production of different biofuels including biodiesel, bio. Heterotrophic cultivation of microalgae for production of. Furthermore, microalgal biodiesel production is restricted mostly due to the high cost of the fermentation substrate, while the cost of glucose accounted for 80% of the total medium cost li et al. Production of biodiesel from microalgae intechopen. The aim of this work was to evaluate the performance of microalgal heterotrophic bioreactors in the secondary treatment of cattleslaughterhouse. Enzymatic production of biodiesel from microalgal oil. Nano 3 sources to induce oil accumulation in its cell and compared to the controlled or natural condition of autotrophic cultivation supplemented with light intensity of. Introduction more oil for biodiesel production than traditional crops on an. Kinetic studies and thermodynamics of oil extraction and.
May 21, 2014 this article presents a study on screening of microalgal strains from the peking university algae collection and heterotrophic cultivation for biodiesel production of a selected microalgal strain. The lipidtomethanol molar ratio sratio is an important parameter for lipid extraction, but its effects on product yield were found insignificant between levels of sratio due to the higher ratios tested in this study. Heterotrophic cultivation of microalgae for production of biodiesel. Pdf on jan 1, 2009, lopes da silva t and others published multiparameter flow cytometry as a tool to monitor heterotrophic microalgal batch fermentations for oil production towards biodiesel. It is possible to produce adequate microalgal biofuels to satisfy the fast growing energy demand within the restraints of land and water resources. Oct 01, 2008 in this work, microgreen algae of chlorella vulgaris tistr 8580 was cultivated under heterotrophic conditions of no light intensity in the dark, carbon 10 g l. This report assesses the economics of microalgae biofuels production. The residual biomass could be either dried and sold as animal feed, similar to the distiller dried grains coproduced with corn ethanol, or equally plausibly, converted to biogas. Biomass yields in heterotrophic and mixotrophic conditions 15. Pdf multiparameter flow cytometry as a tool to monitor.
Multiparameter flow cytometry was used to monitor cell intrinsic light scatter, viability, and lipid content of chlorella protothecoides cells grown in shake flasks. Heterotrophic growth of chlorella protothecoides resulted. For realizing commercial production of biodiesel from heterotrophic c. Microalgal biofuel production is potentially sustainable. Comparison of autotrophic and mixotrophic cultivation of. Heterotrophic culture of microalgae using biodiesel derived glycerol a dissertation presented to the graduate school of clemson university in partial fulfillment of the requirements for the degree doctor of philosophy biosystems engineering. Delft university of technology biodiesel production from. The results suggested that heterotrophic growth of microalgae is an efficient method for the production of biomass and high lipid content in the cells, which can reduce the cost of microalgal biomass production and microalgal oil production. Chlorella protothecoides, biodiesel, symbiosis, autotrophic, heterotrophic 1. Production of single cell oil for biodiesel from microalgae. Pkuac 102 was found the best for the production of heterotrophic algal biodiesel. Biofuel production by oleaginous microalgae is a promising alternative to.
Microalgae has much more oil than macroalgae and it is much faster and easier to grow10. Heterotrophic growth of chlorella protothecoides resulted in the accumulation of high lipid. Introduction more oil for biodiesel production than traditional crops on an nowadays, about 80% of global energy demand is. Microalgal lipid production by microalgae chlorella sp. Cultivation of oily microalgae for the production of third. Biodiesel production from heterotrophic microalgae. Pdf production of biodiesel from microalgae researchgate. The 500 million m 3 of biodiesel required by transportation in the usa would thus require microalgae to be grown on 5. Multiparameter flow cytometry as a tool to monitor. This article presents a study on screening of microalgal strains from the peking university algae collection and heterotrophic cultivation for biodiesel production of a selected microalgal strain. Schizochytrium limacinum is a heterotrophic microalga that is capable of producing high levels of biomass and total fatty acid. Department of bioprocess technology, faculty of biotechnology and biomolecular sciences, universiti putra malaysia, 43400 upm serdang, selangor, malaysia.
The present study introduced an integrated method for the production of biodiesel from microalgal oil. However, the commercial production of microalgal biodiesel was halted due to its high cost. Treatment of cattleslaughterhouse wastewater and the reuse. The objective of this work is to produce heterotrophic microalgal lipid in flaskbatch fermentation. Fermentors are mainly used to produce heterotrophic microalgae using. This dissertation is brought to you for free and open access by washington university open scholarship.
Screening, growth medium optimisation and heterotrophic. Moreover, microalgae can grow in seawater or wastewater and microalgal oil can exceed 5060% dry cell weight as compared with some best agricultural oil crops. The objective of this work is to explore the potential of producing biodiesel fuel from this alga using different biodiesel preparation methods, including oil extraction followed by. However, the microalgal productivity can easily attain 1. Heterotrophic oil production from microalgae greatly exceeds that of vegetable oil crops. Microalgae biodiesel pdf institute of technology and engineering, massey university, private bag 11. High cell density, heterotrophic microalgae, axenic. Biodiesel optimum conversion yield of 63% was obtained with 56. The effect of ethyl acetate on the stability of commercial immobilized lipase, novozym 435, was also investigated. In situ biodiesel production from fastgrowing and high oil.
Biodiesel produced from microalgae will not compromise the production of food and other products derived from crops. Algal companies specialized in biodiesel production the following is a list of companies developing processes for the production of biodiesel from algae oil. These include methane produced by anaerobic digestion of the algal biomass6 biodiesel derived from microalgal oil 11,12, and. The characteristics of acidic transesterification of microalgal oil and the properties of the biodiesel were investigated. Biodiesel production from chlorella protothecoides oil by. Changes in the right angle light scatter rals and forward angle light scatter fals were detected during the microalgal growth, which were attributed to the different microalgal cell cycle stages. Other limitations include the low concentrations of free co 2 that are required for peak algal growth and that algal grazers are a significant, but. Biodiesel production by microalgal biotechnology mafiadoc.
Biodiesel production potential of wastewater microalgae. Heterotrophic cultivation of microalgae for production of biodiesel volume. Progress and challenges in microalgal biodiesel production. Apr 16, 2019 nutrient removal and microalgal biomass production from different anaerobic digestion effluents with chlorella species. Biomass and lipid production of heterotrophic microalgae.
Production of biodiesel from microalgae through mafiadoc. Nutrient removal and microalgal biomass production from. Microalgae have high potentials in biodiesel production compared to other oil crops. The quality of the oil produced from the cells of heterotrophic growth is also superior compared the oil. Biodiesel comparable to conventional diesel was obtained from heterotrophic microalgal oil by acidic transesterification. Global increases in atmospheric co2 and climate change are drawing considerable attention to identify sources of energy with lower environmental impact than those currently in use.
Multiparameter flow cytometry as a tool to monitor heterotrophic microalgal batch fermentations for oil production towards biodiesel. Potential assessment of microalgal oils for biodiesel. Microalgae have high biomass productivity, high lipid content, low cultivation cost, and no competition with agricultural land. Biodiesel production from heterotrophic microalgal oil. Biodiesel lipids fatty acids production abstract biodiesel has received much attention in recent years. We began with the heterotrophic cultivation of auxenochlorella protothecoides in a 60m3 fermentor that produced biomass at 3.
Treatment of cattleslaughterhouse wastewater and the. Biodiesel production by microalgal biotechnology rvo. Heterotrophic culture of microalgae using biodiesel. Fermentors are mainly used to produce heterotrophic microalgae using an organic. Pufa content in the microalgal oils was negligible under the investigated conditions, which confirms the potential of biodiesel production from spirulina. In this paper, the different sources of energy such as oil, conventional and. Biodiesel production by microalgal biotechnology request pdf. First, the cultivation of microalgae dose not need much land as compared to that of terraneous plants. Pkuac 102 was found the best for the production of heterotrophic algal. Ratanaporn leesing, supaporn kookkhunthod, ngarmnit nontaso abstract.
Pdf biodiesel production from heterotrophic microalgal oil. Producing biofuel from microalgae has gained renewed interest recently. Microalgaederived biodiesel has been regarded as a promising alternative for fossil diesel. Miao and wu studied biodiesel production from heterotrophic cultivated microalgae oil from c. Biodiesel production from heterotrophic microalgal oil sciencedirect. In situ transesterification of microalgal oil to produce algal biodiesel 1 executive summary microalgae are promising candidate biomass resources as potential feedstocks for renewable energy production. Heterotrophic growth of chlorella protothecoides resulted in the accumulation of high lipid content 55% in cells. Although numerous reports are available on the production of biodiesel from vegetable oils of terraneous oilplants, such as soybean, sun. Heterotrophic cultivation of microalgae for lipids production. Jul 14, 2009 multiparameter flow cytometry was used to monitor cell intrinsic light scatter, viability, and lipid content of chlorella protothecoides cells grown in shake flasks. When made from seaweed macroalgae it can be known as seaweed fuel or seaweed oil several companies and government agencies are funding efforts to reduce capital. Large amount of microalgal oil was efficiently extracted from these heterotrophic cells by using nhexane.
An acid catalyst hydrochloric acid was used in this research because the alkali catalyst was not suitable for the transesterification of microalgal oil, most likely because of the high acid value of microalgal oil. The economic analysis of jatropha biodiesel revealed the cost of production of jatropha ethyl ester to be 0. Algae fuel, algal biofuel, or algal oil is an alternative to liquid fossil fuels that uses algae as its source of energyrich oils. The best process combination was 100% catalyst quantity based on oil weight with 56. In making biodiesel, triglycerides are reacted with. In this work, microgreen algae of chlorella vulgaris tistr 8580 was cultivated under heterotrophic conditions of no light intensity in the dark, carbon 10 g l. Transesterification reactions were performed by using chlorella protothecoides oil as feedstock, methanol, and potassium hydroxide as the catalyst. Comparison of autotrophic and heterotrophic cultivations of. Further efforts in microalgae screening for biodiesel production are needed to discover a native microalgae that will be feasible for biodiesel production in terms of biomass productivity and oil. Microalgal biotechnology appears to possess high potential for biodiesel production because a significant increase in lipid content of microalgae is now possible through heterotrophic cultivation. The quality of the oil produced from the cells of heterotrophic growth is also superior compared the oil from photoautotrophic growth. Also, algae fuels are an alternative to commonly known biofuel sources, such as corn and sugarcane.
Here, we presented a pilot study on the industrial production of algal biodiesel. Microalgal heterotrophic bioreactors are a potential technological development that can convert organic matter, nitrogen and phosphorus of wastewaters into a biomass suitable for energy production. Converting the algal oil to biofuels biodiesel, green diesel, jet fuel may be the lesser challenge. Production of methyl esters, or biodiesel, from microalgal oil has been demonstrated belarbi et al.
Modeling the production of microalgal biodiesel mark henson washington university in st. Nutrient removal and microalgal biomass production from different anaerobic digestion effluents with chlorella species. Figure 5 effects of temperature and reaction time on the biodiesel yield by in situ transesterification of c. At present, algal oil represents less than 2% of human epadha. Biodiesel production from microalgae lipids can, in the future, occupy a prominent place in energy generation because it represents a sustainable alternative to petroleumbased fuels. In the present study, an integrated method for biodiesel production from heterotrophic microalgal oil by acidic transesterification was developed. However, commercial application of biodiesel production from c. When made from seaweed macroalgae it can be known as seaweed fuel or seaweed oil. May 11, 2007 an integrated approach of biodiesel production from heterotrophic chlorella protothecoides focused on scaling up fermentation in bioreactors was reported in this study. Microalgal feedstocks have been considered a suitable alternative to traditional oil. In this paper, the different sources of energy such as oil, conventional and unconventional. Biodiesel fuel production from algae as renewable energy. High cell density cultivation of microalgae via heterotrophic growth mechanism could effectively address the issues of low productivity and operational constraints presently affecting the solar driven biodiesel production.